Optical Nanoscopy Blog

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Open Access Encyclopedia for Photonics and Laser Technology

This comprehensive open-access encyclopedia, authored by Dr. Rรผdiger Paschotta and provided by RP Photonics Consulting GmbH, explains the physical principles and common techniques in laser technology, while also covering major areas of fiber-optic technology and nonlinear optics, and addressing supplementary topics like ultrashort pulses, optical communications, general optics, optoelectronics, and quantum optics. It contains 619 full articles, not just definitions as in a dictionary or a glossary. Many references to selected scientific articles and textbooks aid further studies. Substantial work has been invested to provide all information with textbook quality. With this work, RP Photonics provides a much appreciated service to both the photonics industry and the academic community.

The encyclopedia is freely accessible via this website. Simply click on one of the letters in the table above in order to display the keywords beginning with that letter. Enjoy ๐Ÿ™‚


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Lectures About Super-Resolution Fluorescence Microscopy & Its Applications by Xiaowei Zhuang (Harvard/HHMI)

Zhuang begins her lecture by explaining that the resolution of traditional light microscopy is about 200 nm due to the diffraction of light. This diffraction limit has long hampered the ability of scientists to visualize individual proteins and sub-cellular structures. The recent development of sub-diffraction limit, or super resolution, microscopy techniques, such as STORM, allows scientists to obtain beautiful images of individual labeled proteins in live cells. In Part 2 of her talk, Zhuang gives two examples of how her lab has used STORM; first to study the chromosome organization of E. coli and second, to determine the molecular architecture of a synapse.

Enjoy the lectures ๐Ÿ™‚


iBiology Microscopy Course

Light microscopy has become one of the most useful tools in the life sciences. Following the traditions of great courses on light microscopy, such as those offered by the Marine Biological Laboratory, EMBO, and the NCBS in Bangalore, this free online comprehensive course begins with the basics of optics, proceeds through transmitted light microscopy, covers the various methods of imaging fluorescent samples, describes how cameras work and image processing, and concludes with some of the latest advances in light microscopy. In addition to lectures, they also provide labs (filmed at a microscope) and short tips, so as to cover pragmatics of how to use microscopes. Assessments are provided for each lecture. Enjoy learning microscopy!


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Method of the Year 2008

With its tremendous potential for understanding cellular biology now poised to become a reality, super-resolution fluorescence microscopy was the choice for Nature Methods’ Method of the Year 2008.

Read the special feature on super-resolution microscopy in Nature Methods, including a Perspective by Stefan Hell and a Commentary by Jennifer Lippincott-Schwartz.

Enjoy the video on YouTube: