Optical Nanoscopy Blog

Lectures About Super-Resolution Fluorescence Microscopy & Its Applications by Xiaowei Zhuang (Harvard/HHMI)

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Zhuang begins her lecture by explaining that the resolution of traditional light microscopy is about 200 nm due to the diffraction of light. This diffraction limit has long hampered the ability of scientists to visualize individual proteins and sub-cellular structures. The recent development of sub-diffraction limit, or super resolution, microscopy techniques, such as STORM, allows scientists to obtain beautiful images of individual labeled proteins in live cells. In Part 2 of her talk, Zhuang gives two examples of how her lab has used STORM; first to study the chromosome organization of E. coli and second, to determine the molecular architecture of a synapse.

Enjoy the lectures 🙂

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